Hit selection using a fixed threshold

Open an assay file
Check the file name
Set the hit selection computation parameters ( Properties and Settings : General & Hit Selection )
Select Hit Selection => Sigma Selection (whole assay) or Sigma Selection (by plates)
Save the file
You can open the results file using a text editor; the hits values are followed by  "*"
You can make a hit list file using Conversion Tools => Hit List from the Hit mtx file